pLKO.1-shLuc.mKO2
(Plasmid #85224)

• Purpose: shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.
• Depositing Lab: Sandra Martha Gomes Dias
• Publication: Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 10.1186/s12885-017-3726-2.

Backbone

• Vector backbone: pLKO.1-TRC.mKO2
• Backbone size w/o insert (bp): 8919
• Total vector size (bp): 7102
• Vector type: Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly Luciferase
• Alt name: Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)
• gRNA/shRNA sequence: TTACGCTGAGTACTTCGA
• Species: Synthetic; Photinus pyralis
• Promoter: RNA polymerase III promoter for human U6 snRNA for shRNA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GACTATCATATGCTTACCGT

Resource Information

• Supplemental Documents:
  • pLKO.1-shLuc.mKO2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector.

Plasmid grows more slowly than standard plasmids.