PH Akt-Venus
(Plasmid #85223)

• Purpose: Venus tagged PH domain of Akt for use as a PIP3 sensor
• Depositing Lab: Narasimhan Gautam
• Publication: O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50. doi: 10.1091/mbc.E15-12-0832. Epub 2016 Mar 3.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6582
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PH AKT(1-148)
• Species: H. sapiens (human)
• Insert Size (bp): 444
• GenBank ID: NM_001014431.1
• Entrez Gene: AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
• Promoter: CMV
• Tag / Fusion Protein:
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH reverse primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry