Skip to main content
Addgene

pRS405-LEU2-TEF-KpolDcr1v2
(Plasmid #85193)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85193 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS405
  • Vector type
    Yeast Expression ; integrating, shuttle vector
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dcr1
  • Alt name
    Dicer from K. polysporus
  • Species
    Kluyveromyces polysporus
  • Promoter TEF

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TEF-seq (5'-ggtcttcaatttctcaagtttc-3')
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS405-LEU2-TEF-KpolDcr1v2 was a gift from David Bartel (Addgene plasmid # 85193 ; http://n2t.net/addgene:85193 ; RRID:Addgene_85193)

  • For your References section:

    Structure of yeast Argonaute with guide RNA. Nakanishi K, Weinberg DE, Bartel DP, Patel DJ. Nature. 2012 Jun 20;486(7403):368-74. doi: 10.1038/nature11211. 10.1038/nature11211 PubMed 22722195
Related items:
Commonly requested with: