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pRS402-GPD-GFP(S65T)
(Plasmid #85190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS402
  • Modifications to backbone
    GFP was amplified from pFA6a-GFP(S65T)-kanMX6 and cloned between GPD promoter and CYC1 terminator
  • Vector type
    Yeast Expression ; integrating, shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Synthetic
  • Promoter GPD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GPDpro-F (5'-CGGTAGGTATTGATTGTAATTCTG-3')
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS402-GPD-GFP(S65T) was a gift from David Bartel (Addgene plasmid # 85190 ; http://n2t.net/addgene:85190 ; RRID:Addgene_85190)

  • For your References section:

    Structure of yeast Argonaute with guide RNA. Nakanishi K, Weinberg DE, Bartel DP, Patel DJ. Nature. 2012 Jun 20;486(7403):368-74. doi: 10.1038/nature11211. 10.1038/nature11211 PubMed 22722195
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