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PurposeExpresses full-length mouse TRPM5 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85189 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRPM5
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GenBank IDAY280364
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Entrez GeneTrpm5 (a.k.a. 9430099A16Rik, LTrpC-5, Ltrpc5, Mtr1)
- Promoter CMV
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-TRPM5 was a gift from Craig Montell (Addgene plasmid # 85189 ; http://n2t.net/addgene:85189 ; RRID:Addgene_85189) -
For your References section:
TRPM5 is a voltage-modulated and Ca(2+)-activated monovalent selective cation channel. Hofmann T, Chubanov V, Gudermann T, Montell C. Curr Biol. 2003 Jul 1;13(13):1153-8. S0960982203004317 [pii] PubMed 12842017
