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Addgene

pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)
(Plasmid #85179)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
  • Backbone size w/o insert (bp) 9530
  • Total vector size (bp) 10280
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nuclear localization signal (nls) tagRFP
  • Species
    SV40, Entacmaea quadricolor
  • Insert Size (bp)
    750

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer EBV_rev_primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone plasmid (pK038) is available in Addgene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova) was a gift from Takuji Iwasato (Addgene plasmid # 85179 ; http://n2t.net/addgene:85179 ; RRID:Addgene_85179)

  • For your References section:

    Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. 10.1038/srep35747 PubMed 27775045
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