pmNFH
(Plasmid #85178)

• Purpose: Mouse neurofilament protein H cDNA mammalian expression construct
• Depositing Lab: Anthony Brown
• Publication: Colakoglu et al J Cell Biol. 2009 Jun 1;185(5):769-77. doi: 10.1083/jcb.200809166. Epub 2009 May 25.

Backbone

• Vector backbone: pEGFP-C1 with EGFP sequence removed
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4344
• Total vector size (bp): 7614
• Modifications to backbone: EGFP sequence removed using Age I and BspE I
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse NFH
• Alt name: mNFH
• Alt name: neurofilament protein H
• Species: M. musculus (mouse)
• Insert Size (bp): 3270
• GenBank ID:
• Entrez Gene: Nefh (a.k.a. NF-H, NF200, Nfh, mKIAA0845)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please cite Çolakoğlu & Brown (2009), "Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing", Journal of Cell Biology 185: 769-777. This plasmid was used in that study but was inadvertently not described in the Methods.