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Purpose(Empty Backbone) Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
| Cloning Grade DNA | 85168-DNA.cg | 2 µg of cloning grade DNA in Tris buffer | 1 | $105 * | |
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Backbone
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Vector backbonepBBR1MCS
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Modifications to backboneswapped antibiotic resistance cassette
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Vector typeBacterial Expression
- Promoter Constitutive (derepressed P-lac)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Vector is mobilisable via RP4/RK2 mating system, e.g. using E.coli strains S17 or SM10. Note that cloned genes are expressed from the lac promoter, but that this is not controllable with IPTG, due to absence of lacI repressor gene on the plasmid. (chromosomal lacI does not provide sufficient repression).
MCS: Has unique KpnI, ApaI, XhoI, HincII, SalI, ClaI, HindIII, EcoRV, EcoRI, SmaI, BamHI, SpeI, XbaI, SacII, SacI sites for cloning
Information for Cloning Grade DNA (Catalog # 85168-DNA.cg) ( Back to top)
Purpose
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
Delivery
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $105 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBBR1MCS-2 was a gift from Kenneth Peterson (Addgene plasmid # 85168 ; http://n2t.net/addgene:85168 ; RRID:Addgene_85168) -
For your References section:
Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM 2nd, Peterson KM. Gene. 1995 Dec 1;166(1):175-6. 0378111995005841 [pii] PubMed 8529885
