pCIpA102-G-HLA-A2_GFP
(Plasmid #85162)

• Purpose: for mRNA expression of HLA.A2-EGFP
• Depositing Labs: Tone Fredsvik Gregers, Sébastien Walchli
• Publication: Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. doi: 10.1002/eji.201343671. Epub 2013 Dec 27.

Backbone

• Vector backbone: pCIpA102-GTW(JO249)
• Vector type: Mammalian Expression ; mRNA expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HLA.A2
• Species: H. sapiens (human)
• Entrez Gene: HLA-A (a.k.a. HLAA)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: It was cloned from the cDNA of an healthy donor and sequence confirmed as HLA-A*0201
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCI-A102 constructs were opened with MfeI and used as a template for small-scale mRNA synthesis