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Addgene

pENTR-HLA-A2_GFP
(Plasmid #85161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR
  • Total vector size (bp) 4407
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HLA.A2
  • Species
    H. sapiens (human)
  • Entrez Gene
    HLA-A (a.k.a. HLAA)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    It was cloned from the cDNA of an healthy donor and sequence confirmed as HLA-A*0201

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-HLA-A2_GFP was a gift from Tone Fredsvik Gregers & Sébastien Walchli (Addgene plasmid # 85161 ; http://n2t.net/addgene:85161 ; RRID:Addgene_85161)

  • For your References section:

    Invariant chain as a vehicle to load antigenic peptides on human MHC class I for cytotoxic T-cell activation. Walchli S, Kumari S, Fallang LE, Sand KM, Yang W, Landsverk OJ, Bakke O, Olweus J, Gregers TF. Eur J Immunol. 2014 Mar;44(3):774-84. doi: 10.1002/eji.201343671. Epub 2013 Dec 27. 10.1002/eji.201343671 PubMed 24293164
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