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Addgene

HOXA10 (human) HIS-tag pET
(Plasmid #8516)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28c
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA10
  • Alt name
    HOX1H
  • Alt name
    HOX1.8
  • Alt name
    PL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1820
  • GenBank ID
    S41209
  • Entrez Gene
    HOXA10 (a.k.a. HOX1, HOX1.8, HOX1H, PL)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII blunt (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.
Amino acid sequence differs from initial genbank entry - matches AF040714 (see attached)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA10 (human) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8516 ; http://n2t.net/addgene:8516 ; RRID:Addgene_8516)

  • For your References section:

    A human Hox 1 homeobox gene exhibits myeloid-specific expression of alternative transcripts in human hematopoietic cells. Lowney P, Corral J, Detmer K, LeBeau MM, Deaven L, Lawrence HJ, Largman C. Nucleic Acids Res 1991 Jun 25;19(12):3443-9. 10.1093/nar/19.12.3443 PubMed 1676505
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