HOXA9 (murine) FLAG MSCV
(Plasmid #8515)

• Depositing Lab: Corey Largman
• Publication: Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Backbone

• Vector backbone: MIG (MSCV IRES GFP)
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXA9
• Alt name: HOX1G
• Alt name: HOX1.7
• Species: M. musculus (mouse)
• Insert Size (bp): 1066
• Mutation: makes GFP from an IRES driving translation from a single mRNA
• GenBank ID: NM_010456
• Entrez Gene: Hoxa9 (a.k.a. D6a9, Hox-1.7)
• Tags / Fusion Proteins:
  • FLAG (DYKDDDDK) (N terminal on insert)
  • IRES-GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN5'

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene verified sequence confirms that the FLAG sequence is DYKDDDDK.