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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8513 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneprk5
- Backbone size w/o insert (bp) 4716
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRaptor
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SpeciesH. sapiens (human)
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Insert Size (bp)4071
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Entrez GeneRPTOR (a.k.a. KOG1, Mip1)
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Tag
/ Fusion Protein
- ha (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene has compiled full sequence for this plasmid. Please see Notes link.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA Raptor was a gift from David Sabatini (Addgene plasmid # 8513 ; http://n2t.net/addgene:8513 ; RRID:Addgene_8513) -
For your References section:
mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Cell 2002 Jul 26;110(2):163-75. 10.1016/S0092-8674(02)00808-5 PubMed 12150925
Map uploaded by Addgene staff.
