pAM5089
(Plasmid
#85125)
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PurposeKaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneKaiB expressed from native promoter from NS2-Nt.
- Total vector size (bp) 4516
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Nourseothricin (clonNat), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePkaiB-kaiB
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SpeciesSynechoccus elongatus PCC 7942
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Insert Size (bp)764
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM5089 was a gift from Susan Golden (Addgene plasmid # 85125 ; http://n2t.net/addgene:85125 ; RRID:Addgene_85125) -
For your References section:
Dynamic localization of the cyanobacterial circadian clock proteins. Cohen SE, Erb ML, Selimkhanov J, Dong G, Hasty J, Pogliano J, Golden SS. Curr Biol. 2014 Aug 18;24(16):1836-44. doi: 10.1016/j.cub.2014.07.036. Epub 2014 Aug 7. 10.1016/j.cub.2014.07.036 PubMed 25127213