pAM4645
(Plasmid
#85124)
-
PurposeNSII vector expressing PkaiB-KaiBC. Nt resistant. Complements rhthyms in AMC705
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 85124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneNSII vector expressing PkaiB-KaiBC
- Total vector size (bp) 5900
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Nourseothricin (clonNat), 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePkaiBC-KaiBC
-
SpeciesSynechoccus elongatus PCC 7942
-
Insert Size (bp)2378
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAM4645 was a gift from Susan Golden (Addgene plasmid # 85124 ; http://n2t.net/addgene:85124 ; RRID:Addgene_85124) -
For your References section:
Dynamic localization of the cyanobacterial circadian clock proteins. Cohen SE, Erb ML, Selimkhanov J, Dong G, Hasty J, Pogliano J, Golden SS. Curr Biol. 2014 Aug 18;24(16):1836-44. doi: 10.1016/j.cub.2014.07.036. Epub 2014 Aug 7. 10.1016/j.cub.2014.07.036 PubMed 25127213