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Addgene

pAM5085
(Plasmid #85122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
  • Total vector size (bp) 6181
  • Vector type
    Bacterial Expression
  • Selectable markers
    Streptomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
  • Species
    Synechoccus elongatus PCC 7942
  • Insert Size (bp)
    1904
  • Tag / Fusion Protein
    • ECFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM5085 was a gift from Susan Golden (Addgene plasmid # 85122 ; http://n2t.net/addgene:85122 ; RRID:Addgene_85122)

  • For your References section:

    Dynamic localization of the cyanobacterial circadian clock proteins. Cohen SE, Erb ML, Selimkhanov J, Dong G, Hasty J, Pogliano J, Golden SS. Curr Biol. 2014 Aug 18;24(16):1836-44. doi: 10.1016/j.cub.2014.07.036. Epub 2014 Aug 7. 10.1016/j.cub.2014.07.036 PubMed 25127213
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