pYES260-sc.eEF2 K509A Sequences (3)

Addgene Sequences:

Full (1)
Partial (2)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

Analyze Sequence

GenBank

SnapGene

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> Addgene Assembled Sequence AAAACAGGAAGATTGTATAAGCAAATATTTAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAA TTTTTGTTAAATCAGCTCATTTTTTAACGAATAGCCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAA TAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTCCAACAAGAGTCCACTATTAAAGAACGTGGACTCCA ACGTCAAAGGGCGAAAAAGGGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTT TTTGGGGTCGAGGTGCCGTAAAGCAGTAAATCGGAAGGGTAAACGGATGCCCCCATTTAGAGCTTGACGG GGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGGGCTAGGGCGGTGGGAA GTGTAGGGGTCACGCTGGGCGTAACCACCACACCCGCCGCGCTTAATGGGGCGCTACAGGGCGCGTGGGG ATGATCCACTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTG CGTCCTCGTCCTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAA AGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTT CAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGT AATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATTAACCACT TTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAA AATTGTTAATATACCTCTATACTTTAACGTCAAGGAGAAAAAACCCCGGATCGGACTACTAGCAGCTGTA ATACGACTCACTATAGGGAATATTAAGCTTATGCACCATCACCATCACCATGCTAGCGAGAATCTTTATT TTCAGGGCGCCATGGAGGttgctttcactgttgaccaaatgcgttctttaatggacaaagttaccaatgt gcgtaacatgtccgttattgctcacgtcgatcatggtaagtccactttgaccgattccttggtccaaaga gccggtattatttccgctgctaaggctggtgaagctcgtttcaccgataccagaaaggatgaacaagaaa gaggtatcactatcaagtctaccgctatttctctatactctgaaatgtctgacgaagatgtcaaggaaat caagcaaaagaccgacggtaactccttcttgatcaacttgatcgactctccaggtcacgttgacttctcc tctgaagttactgccgctttacgtgtcactgacggtgctttggttgtcgtcgacaccattgaaggtgtct gtgtccaaaccgaaactgttttgagacaagctttgggtgaGagaatcaagcctgttgttgttatcaacaa ggtcgacagagctttgttggaattgcaagtttctaaggaagatttataccaaacctttgccagaactgtt gaatccgttaacgtcatcgtttccacctacgccgatgaagttttgggtgatgtccaagtttacccagcca gaggtaccgttgccttcggttccggtttgcacggttgggctttcactatccgtcaattcgccaccagata tgctaagaaattcggtgtcgacaaggccaagatgatggacagattatggggtgactctttcttcaaccca aagaccaagaagtggaccaacaaggacactgatgctgaaggtaagccattggaaagagctttcaacatgt tcatcttggacccaatcttcagattattcactgctatcatgaacttcaagaaagaTgaaattccagtttt gctagaaaagttggaaattgtcttgaagggtgacgaaaaggacttggaaggtaaggccttgttgaaggtt gttatgagaaagttcttgccagctgccgatgccttattggaaatgattgtcttgcacttgccatctccag tcactgctcaagcctacagagctgaacaattatacgaaggtccagctgacgatgccaactgtattgctat caagaactgtgatccaaaggctgatttgatgttgtacgtctccaagatggtgccaacctctgataagggt agattctacgccttcggtagagtttttgccggtactgttaagtccggtcaaaaggtcagaatccaaggtc caaactacgttccaggtaagaaggacgatttgttcatcaaggccattcaaagagttgttttgatgatggg tagatttgtcgaaccaatcgatgactgtccagccggtaacattatcggtttagtcggtatcgatcaattc ttgttgaagactggtactttgaccaccagtgaaactgctcacaacatgaaggtcatgaaattctctgtct ctccagttgtgcaagtcgctgtcgaagtcaagaacgctaacgacttaccaaaattggtcgaaggtttgGC gagattgtccaagtctgatccatgtgtcttgacctatatgtctgaatccggtgaacatatcgttgctggt accggtgaattgcatttggaaatttgtttgcaagatttggaacacgaccacgctggtgttccattgaaga tctccccaccagttgtcgcttacagagaaactgttgaaagtgaatcttctcaaactgctttgtccaagtc tccaaacaagcataacagaatctacttgaaggctgaaccaattgacgaagaagtctctttggctattgaa aacggtatcatcaacccaagagatgatttcaaggccagagctagaatcatggctgacgactacggttggg atgtcaccgatgccagaaagatctggtgtttcggtccagacggtaacggtccaaacttggttattgacca aactaaggctgtccaatacttgcacgaaatcaaggattccgttgttgctgctttccaatgggctaccaag gaaggtccaattttcggtgaagaaatgagatctgtcagagttaacattttggatgttactttacatgccg atgctatccacagaggtggtggtcaaatcatcccaaccatgagaagagctacttacgccggtttcttgtt ggctgatccaaagatccaagaaccagttttcttggtcgaaattcaatgtccagaacaagccgtcggtggt atctactccgtcttaaacaagaagagaggtcaagtcgtttctgaagaacaaagaccaggtactccattgt ttaccgtcaaggcctacttgccagttaacgaatctttcggtttcactggtgaattgagacaagctaccgg tggtcaagctttcccacaaatggttttcgaccattggtccactttaggttctgacccattggacccaacc tctaaggctggtgaaattgttcttgctgctcgtaagagacacggtatgaaggaagaagttccaggctggc aagaatattacgacaaattgtaaGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGATATC CATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCGCATCATGTAATTAGTTATGTCACGCTTAC ATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCC TATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTG TACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGG CTTTAATTTGCGGCCCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCT CTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTC AAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAG CAAAAGCCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCC CCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTC CCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCT TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCG AGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACA AACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAA GAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTA AAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATC TGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGCGCTTAC CATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAA CCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATTCAGTCTATTAAT TGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGGCATTGCTACAG GCATCGTGGTGTCACTCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGT TACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAG TTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAA GATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTG CTCTTGCCCGGCGTCAATACGGGATAATAGTGTATCACATAGCAGAACTTTAAAAGTGCTCATCATTGGA AAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTC GTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCA AAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATGG GTAATAACTGATATAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATAC AGTTTTTTAGTTTTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCCTGCTTTTCTGTAACGTTCACCC TCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAATAATAATGTCAGATCCTGTAGAGA CCACATCATCCACGGTTCTATACTGTTGACCCAATGCGTCTCCCTTGTCATCTAAACCCACACCGGGTGT CATAATCAACCAATCGTAACCTTCATCTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAA TCTTTGTCGCTCTTCGCAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATG ACAATTCTGCTAACATCAAAAGGCCTCTAGGTTCCTTTGTTACTTCTTCTGCCGCCTGCTTCAAACCGCT AACAATACCTGGGCCCACCACACCGTGTGCATTCGTAATGTCTGCCCATTCTGCTATTCTGTATACACCC GCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTCTGTCTTCGAAGAGTAAAAAATTGT ACTTGGCGGATAATGCCTTTAGCGGCTTAACTGTGCCCTCCATTGAAAAATCAGTCAAGATATCCACATG TGTTTTTAGTAAACAAATTTTGGGACCTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACA TCCAATGAAGCACACAAGTTTGTTTGCTTTTCGTGCATGATATTAAATAGCTTGGCAGCAACAGGACTAG GATGAGTAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTCGTTTCCTGCAGGTTTTTGT TCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGTTTCTTCAACACTACATATGCGTATATATACC AATCTAAGTCTGTGCTCCTTCCTTCGTTCTTCCTTCTGTTCGGAGATTACCGAATCAAAAAAATTTCAAA GAAACCGAAATCAAAAAAAAGAATAAAAAAAAAATGATGAATTGAATTGAAAAGCTAGCTTATCGATGAT AAGCTGTCAAAGATGAGAATTAATTCCACGGACTATAGACTATACTAGATACTCCGTCTACTGTACGATA CACTTCCGCTCAGGTCCTTGTCCTTTAACGAGGCCTTACCACTCTTTTGTTACTCTATTGATCCAGCTCA GCAAAGGCAGTGTGATCTAAGATTCTATCTTCGCGATGTAGTAAAACTAGCTAGACCGAGAAAGAGACTA GAAATGCAAAAGGCACTTCTACAATGGCTGCCATCATTATTATCCGATGTGACGCTGCAGCTTCTCAATG ATATTCGAATACGCTTTGAGGAGATACAGCCTAATATCCGACAAACTGTTTTACAGATTTACGATCGTAC TTGTTACCCATCATTGAATTTTGAACATCCGAACCTGGGAGTTTTCCCTGAAACAGATAGTATATTTGAA CCTGTATAATAATATATAGTCTAGCGCTTTACGGAAGACAATGTATGTATTTCGGTTCCTGGAGAAACTA TTGCATCTATTGCATAGGTAATCTTGCACGTCGCATCCCCGGTTCATTTTCTGCGTTTCCATCTTGCACT TCAATAGCATATCTTTGTTAACGAAGCATCTGTGCTTCATTTTGTAGAACAAAAATGCAACGCGAGAGCG CTAATTTTTCAAACAAAGAATCTGAGCTGCATTTTTACAGAACAGAAATGCAACGCGAAAGCGCTATTTT ACCAACGAAGAATCTGTGCTTCATTTTTGTAAAACAAAAATGCAACGCGACGAGAGCGCTAATTTTTCAA ACAAAGAATCTGAGCTGCATTTTTACAGAACAGAAATGCAACGCGAGAGCGCTATTTTACCAACAAAGAA TCTATACTTCTTTTTTGTTCTACAAAAATGCATCCCGAGAGCGCTATTTTTCTAACAAAGCATCTTAGAT TACTTTTTTTCTCCTTTGTGCGCTCTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAA GGTTAGAAGAAGGCTACTTTGGTGTCTATTTTCTCTTCCATAAAAAAAGCCTGACTCCACTTCCCGCGTT TACTGATTACTAGCGAAGCTGCGGGTGCATTTTTTCAAGATAAAGGCATCCCCGATTATATTCTATACCG ATGTGGATTGCGCATACTTTGTGAACAGAAAGTGATAGCGTTGATGATTCTTCATTGGTCAGAAAATTAT GAACGGTTTCTTCTATTTTGTCTCTATATACTACGTATAGGAAATGTTTACATTTTCGTATTGTTTTCGA TTCACTCTATGAATAGTTCTTACTACAATTTTTTTGTCTAAAGAGTAATACTAGAGATAAACATAAAAAA TGTAGAGGTCGAGTTTAGATGCAAGTTCAAGGAGCGAAAGGTGGATGGGTAGGTTATATAGGGATATAGC ACAGAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTCGCAATGGGAAGCTCC ACCCCGGTTGATAATCAGAAAAGCCCCA

Partial Sequences from Addgene (2)

Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence.

Analyze Sequence

GenBank

SnapGene

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> TEVprot-F 5'-gagaatctttattttcagggc -3' TGTTGACcAaATGCGTTCTTTAATGGACAAAGTTACCAATGTGCGTAACATGTCCGTTATTGCTCACGTC GATCATGGTAAGTCCACTTTGACCGATTCCTTGGTCCAAAGAGCCGGTATTATTTCCGCTGCTAAGGCTG GTGAAGCTCGTTTCACCGATACCAGAAAGGATGAACAAGAAAGAGGTATCACTATCAAGTCTACCGCTAT TTCTCTATACTCTGAAATGTCTGACGAAGATGTCAAGGAAATCAAGCAAAAGACCGACGGTAACTCCTTC TTGATCAACTTGATCGACTCTCCAGGTCACGTTGACTTCTCCTCTGAAGTTACTGCCGCTTTACGTGTCA CTGACGGTGCTTTGGTTGTCGTCGACACCATTGAAGGTGTCTGTGTCCAAACCGAAACTGTTTTGAGACA AGCTTTGGGTGAGAGAATCAAGCCTGTTGTTGTTATCAACAAGGTCGACAGAGCTTTGTTGGAATTGCAA GTTTCTAAGGAAGATTTATACCAAACCTTTGCCAGAACTGTTGAATCCGTTAACGTCATCGTTTCCACCT ACGCCGATGAAGTTTTGGGTGATGTCCAAGTTTACCCAGCCAGAGGTACCGTTGCCTTCGGTTCCGGTTT GCACGGTTGGGCTTTCACTATCCGTCAATTCGCCACCAGATATGCTAAGAAATTCGGTGTCGACAAGGCC AAGATGATGGACAGATTATGGGGTGACTCTTTCTTCAACCCAAAGACCAAGAAGTGGACCAACAAGGACA CTGATGCTGAAGGTAAGCCATTGGAAAGAGCTTTCAACATGTTCATCTTGGACCCAATCTTCAGATTATT CACTGCTATCATGAACTTCAaGAAAGATGAAATTCCAGTTTTGCTAGAaAAGTTGGAAA
Analyze Sequence

GenBank

SnapGene

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> yEEF2-F 5'-caatcgatgactgtccagcc-3' TCGGTATCGATCAATTCTTGTTGAAGACTGGTACTTTGACCACCAGTGAAACTGCTCACAACATGAAGGT CATGAAATTCTCTGTCTCTCCAGTTGTGCAAGTCGCTGTCGAAGTCAAGAACGCTAACGACTTACCAAAA TTGGTCGAAGGTTTGGCGAGATTGTCCAAGTCTGATCCATGTGTCTTGACCTATATGTCTGAATCCGGTG AACATATCGTTGCTGGTACCGGTGAATTGCATTTGGAAATTTGTTTGCAAGATTTGGAACACGACCACGC TGGTGTTCCATTGAAGATCTCCCCACCAGTTGTCGCTTACAGAGAAACTGTTGAAAGTGAATCTTCTCAA ACTGCTTTGTCCAAGTCTCCAAACAAGCATAACAGAATCTACTTGAAGGCTGAACCAATTGACGAAGAAG TCTCTTTGGCTATTGAAAACGGTATCATCAACCCAAGAGATGATTTCAAGGCCAGAGCTAGAATCATGGC TGACGACTACGGTTGGGATGTCACCGATGCCAGAAAGATCTGGTGTTTCGGTCCAGACGGTAACGGTCCA AACTTGGTTATTGACCAAACTAAGGCTGTCCAATACTTGCACGAAATCAAGGATTCCGTTGTTGCTGCTT TCCAATGGGCTACCAAGGAAGGTCCAATTTTCGGTGAAGAAATGAGATCTGTCAGAGTTAACATTTTGGA TGTTACTTTACATGCCGATGCTATCCACAGAGGTGGTGGTCAAATCATCCCAACCATGAGAAGAGCTACT TACGCCGGTTTCTTGTTGGCTGATCCAAAGATCCAAGAACCAGTTTTCTTGGTCGAAATTCAATGTCCAG AACAAGCCGTCGGTGGTATCTACTCCGTCTTAAACAAGAAGAGAGGTCAAGTCGTTTCTGAAGAACAAAG ACCAGGTACTCCATTGTTTACCGTCAAGGCCTACTTGCCAGTTAACGAATCTTTCGGTTTCACTGGTGAA TTGAGACAAGCTACCGG

pYES260-sc.eEF2 K509A Sequences (3)

Full Sequences from Addgene (1)

Partial Sequences from Addgene (2)

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