pF709-pET28a-Hs-FL-ETFbeta-NHis wt
(Plasmid #85110)

• Purpose: expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag
• Depositing Lab: Pal Falnes
• Publication: Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115. Epub 2014 Nov 21.

Backbone

• Vector backbone: pET28a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: human full-length ETFbeta
• Species: H. sapiens (human)
• Insert Size (bp): 768
• Entrez Gene: ETFB (a.k.a. FP585, MADD)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: ATGCGTCCGGCGTAGAGG
• 3′ sequencing primer: TAGAGGCCCCAAGGGGTTATGCTAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Homo sapiens wt ETF beta, full-length, cloned into NdeI-HindIII sites of pET28a, contains a N-terminal His-tag