hAur-A D274A
(Plasmid #8511)

• Depositing Lab: Joan Ruderman
• Publication: Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83. Epub 2004 Nov 9.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hAur-A
• Alt name: Aurora A
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: D274A, catalytically dead mutant
• Entrez Gene: AURKA (a.k.a. AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map. For production of retrovirus expressing human Aur-A mutant. (Ruderman RC233)