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Addgene

pBsaHFm
(Plasmid #85103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85103 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHSG398
  • Backbone manufacturer
    TaKaRa
  • Backbone size w/o insert (bp) 2227
  • Total vector size (bp) 4217
  • Modifications to backbone
    N/A
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Heme-FMN domain of P450 BM3
  • Alt name
    Heme-FMN domain of CYP102A1
  • Species
    Bacillus megaterium
  • Insert Size (bp)
    1990
  • Mutation
    A74G
  • GenBank ID
    WP_034650526.1

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

P450 BM3 was originally cloned from Bacillus megaterium (NBRC 15308) in our lab

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBsaHFm was a gift from Teruyuki Nagamune (Addgene plasmid # 85103 ; http://n2t.net/addgene:85103 ; RRID:Addgene_85103)

  • For your References section:

    Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support. Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T. Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033. 10.1002/anie.201608033 PubMed 27781345
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