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Addgene

pBsaP450camGG
(Plasmid #85101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHSG398
  • Backbone manufacturer
    TaKaRa
  • Backbone size w/o insert (bp) 2227
  • Total vector size (bp) 3498
  • Modifications to backbone
    N/A
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P450cam
  • Alt name
    CYP101A1
  • Species
    Synthetic; Pseudomonas putida
  • Insert Size (bp)
    1271
  • GenBank ID
    AAA25760.1

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A synthetic gene of P450cam was inserted into pHSG398 using In-Fusion cloning kit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBsaP450camGG was a gift from Teruyuki Nagamune (Addgene plasmid # 85101 ; http://n2t.net/addgene:85101 ; RRID:Addgene_85101)

  • For your References section:

    Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support. Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T. Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033. 10.1002/anie.201608033 PubMed 27781345
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