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Addgene

pBsaPro5X
(Plasmid #85100)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHSG398
  • Backbone manufacturer
    TaKaRa
  • Backbone size w/o insert (bp) 2210
  • Total vector size (bp) 2592
  • Modifications to backbone
    N/A
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Putidaredoxin
  • Alt name
    (Pro)5(Gly)4Ser-PdX
  • Species
    Synthetic; Pseudomonas putida
  • Insert Size (bp)
    382
  • Mutation
    changed Cysteine 73 to Serine and Cysteine 85 to Serine
  • GenBank ID
    BAA00414.1

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A synthetic gene of PdX was inserted between EcoRI and BamHI sites of pHSG398 using In-Fusion cloning kit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBsaPro5X was a gift from Teruyuki Nagamune (Addgene plasmid # 85100 ; http://n2t.net/addgene:85100 ; RRID:Addgene_85100)

  • For your References section:

    Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support. Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T. Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033. 10.1002/anie.201608033 PubMed 27781345
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