pGS-3R112C/T180C
(Plasmid #85095)

• Purpose: Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)
• Depositing Lab: Teruyuki Nagamune
• Publication: Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.

Backbone

• Vector backbone: pET-28b(+)
• Backbone manufacturer: Merck Millipore
• Backbone size w/o insert (bp): 5332
• Total vector size (bp): 6125
• Modifications to backbone: N/A
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DNA polymerase sliding clamp 3
• Alt name: PCNA3
• Species: Synthetic; Sulfolobus solfataricus P2
• Insert Size (bp): 793
• Mutation: changed Arginine 112 to Cysteine and Threonine 180 to Cysteine
• GenBank ID: WP_009988781.1
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • His6 tag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PCNA3 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.