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p3R112C/T180CHFm
(Plasmid #85091)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28b(+)
  • Backbone manufacturer
    Merck Millipore
  • Backbone size w/o insert (bp) 5333
  • Total vector size (bp) 8081
  • Modifications to backbone
    N/A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Supplement with 1% glucose is recommended to suppress leaky expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
  • Alt name
    PCNA3(R112C/T180C)-Heme FAD
  • Species
    Synthetic; Sulfolobus solfataricus P2, Bacillus megaterium
  • Insert Size (bp)
    2748
  • Mutation
    R112C/T180C in PCNA3, A74G in heme-FMN domain of P450BM3
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His6 tag (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid was obtained from p3R112C/T180C-GG (Plasmid #85093) and pBsaHFm (Plasmid #85103) by Golden Gate assembly.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3R112C/T180CHFm was a gift from Teruyuki Nagamune (Addgene plasmid # 85091 ; http://n2t.net/addgene:85091 ; RRID:Addgene_85091)

  • For your References section:

    Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support. Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T. Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033. 10.1002/anie.201608033 PubMed 27781345
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