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Addgene

hCdc20 (1-120)
(Plasmid #8509)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8509 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hCdc20 (1-120)
  • Alt name
    Cdc20
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    449
  • Mutation
    Cdc20 N-terminus. Dominant Negative mutant.
  • Entrez Gene
    CDC20 (a.k.a. CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. For production of retrovirus expressing human Cdc20 N-terminus. (Ruderman RC231)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCdc20 (1-120) was a gift from Joan Ruderman (Addgene plasmid # 8509 ; http://n2t.net/addgene:8509 ; RRID:Addgene_8509)

  • For your References section:

    Requirements for the destruction of human Aurora-A. Crane R, Kloepfer A, Ruderman JV. J Cell Sci 2004 Dec 1;117(Pt 25):5975-83. Epub 2004 Nov 9. 10.1242/jcs.01418 PubMed 15536123
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