pR1G108CP20X
(Plasmid #85086)

• Purpose: Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli
• Depositing Lab: Teruyuki Nagamune
• Publication: Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.

Backbone

• Vector backbone: pET-28b(+)
• Backbone manufacturer: Merck Millipore
• Backbone size w/o insert (bp): 5332
• Total vector size (bp): 6499
• Modifications to backbone: N/A
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Supplement with 1% glucose is recommended to suppress leaky expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
• Alt name: PdR-PCNA1(G108C)-(Pro)20-PdX
• Species: Synthetic; Sulfolobus solfataricus P2, Pseudomonas putida
• Insert Size (bp): 2442
• Mutation: G108C in PCNA1
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • His6 tag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A synthetic gene of PdR was inserted at the NdeI site of p1G108CP20X (Plasmid #85096) using In-Fusion cloning kit.