pP450cam
(Plasmid
#85085)
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PurposeExpresses P450cam (CYP101A1) in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28b(+)
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Backbone manufacturerMerck Millipore
- Backbone size w/o insert (bp) 5333
- Total vector size (bp) 6581
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Modifications to backboneN/A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameP450cam
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Alt nameCYP101A1
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SpeciesSynthetic; Pseudomonas putida
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Insert Size (bp)1248
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GenBank IDAAA25760.1
- Promoter T7 promoter
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Tag
/ Fusion Protein
- His6 tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The P450cam gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pP450cam was a gift from Teruyuki Nagamune (Addgene plasmid # 85085 ; http://n2t.net/addgene:85085 ; RRID:Addgene_85085) -
For your References section:
Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support. Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T. Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033. 10.1002/anie.201608033 PubMed 27781345