pCMV-BLZinCh-1
(Plasmid
#85082)
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Purposemammalian vector for the expression of a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBL-ZinCh-1
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site Xho1 (unknown if destroyed)
- 5′ sequencing primer CMV forward primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-BLZinCh-1 was a gift from Maarten Merkx (Addgene plasmid # 85082 ; http://n2t.net/addgene:85082 ; RRID:Addgene_85082) -
For your References section:
Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc. Aper SJ, Dierickx P, Merkx M. ACS Chem Biol. 2016 Oct 21;11(10):2854-2864. Epub 2016 Sep 1. 10.1021/acschembio.6b00453 PubMed 27547982
