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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8508 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABE-puro
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehCdh1 (1-125)
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Alt namehCdh1 N terminus
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Alt nameFzr1
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SpeciesH. sapiens (human)
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Insert Size (bp)464
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MutationCdh1 N-terminus. Dominant Negative mutant.
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Entrez GeneFZR1 (a.k.a. CDC20C, CDH1, DEE109, FZR, FZR2, HCDH, HCDH1)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map. For production of retrovirus expressing human Cdh1 N-terminus. (Ruderman RC230)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hCdh1 (1-125) was a gift from Joan Ruderman (Addgene plasmid # 8508 ; http://n2t.net/addgene:8508 ; RRID:Addgene_8508) -
For your References section:
Requirements for the destruction of human Aurora-A. Crane R, Kloepfer A, Ruderman JV. J Cell Sci 2004 Dec 1;117(Pt 25):5975-83. Epub 2004 Nov 9. 10.1242/jcs.01418 PubMed 15536123
Map uploaded by the depositor.