-
Purpose(Empty Backbone) expression in Pichia pastoris
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGAPZB
-
Backbone manufacturerInvitrogen
- Backbone size (bp) 3000
-
Vector typeYeast Expression
- Promoter GAP
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GAP
- 3′ sequencing primer 3 AOX1 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGKB was a gift from Sheng Yang (Addgene plasmid # 85077 ; http://n2t.net/addgene:85077 ; RRID:Addgene_85077) -
For your References section:
Hygromycin-resistance vectors for gene expression in Pichia pastoris. Yang J, Nie L, Chen B, Liu Y, Kong Y, Wang H, Diao L. Yeast. 2014 Apr;31(4):115-25. PubMed 24822243
