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Addgene

pAHYB-GFP
(Plasmid #85074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAHYB
  • Backbone manufacturer
    Sheng Yang lab
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    800
  • Promoter AOX1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI/SpeI (destroyed during cloning)
  • 5′ sequencing primer 5 AOX1
  • 3′ sequencing primer 3 AOX1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAHYB-GFP was a gift from Sheng Yang (Addgene plasmid # 85074 ; http://n2t.net/addgene:85074 ; RRID:Addgene_85074)

  • For your References section:

    Hygromycin-resistance vectors for gene expression in Pichia pastoris. Yang J, Nie L, Chen B, Liu Y, Kong Y, Wang H, Diao L. Yeast. 2014 Apr;31(4):115-25. PubMed 24822243
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