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Addgene

UbcH10 DN AU1
(Plasmid #8507)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8507 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJS55
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcH10
  • Alt name
    ubiquitin-conjugating enzyme H10
  • Alt name
    E2-C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    561
  • Mutation
    C114S
  • Entrez Gene
    UBE2C (a.k.a. UBCH10, dJ447F3.2)
  • Tag / Fusion Protein
    • AU1 (DTYRYI) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. Expression of tagged UbcH10 C114S mutant protein in mammalian cells from early SV40 promoter. Antibodies to AU1 epitope available from BABCO. (Ruderman pJHEAUC-CS2)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbcH10 DN AU1 was a gift from Joan Ruderman (Addgene plasmid # 8507 ; http://n2t.net/addgene:8507 ; RRID:Addgene_8507)

  • For your References section:

    Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase. Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV. Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2362-7. 10.1073/pnas.94.6.2362 PubMed 9122200
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