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PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1
(Plasmid #85069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3994
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PH-PLCD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3100
  • Mutation
    PLCD aa 1–170
  • GenBank ID
    NP_006216.2
  • Entrez Gene
    PLCD1 (a.k.a. NDNC3, PLC-III)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mScarlet (C terminal on backbone)
    • IRES-sYFP2-PH (C terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AGGTCTATATAAGCAGAGC
  • 3′ sequencing primer TCTACAAATGTGGTATGGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1 was a gift from Dorus Gadella (Addgene plasmid # 85069 ; http://n2t.net/addgene:85069 ; RRID:Addgene_85069)

  • For your References section:

    mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr. Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. 10.1038/nmeth.4074 PubMed 27869816
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