pmScarlet-H_peroxisome_C1
(Plasmid
#85064)
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PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepmScarlet-H_C1
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Backbone manufacturerGadella Lab
- Backbone size w/o insert (bp) 4659
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePeroxisomal targeting sequence
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Alt nameSRL
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SpeciesH. sapiens (human)
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Insert Size (bp)3
- Promoter CMV
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Tag
/ Fusion Protein
- mScarlet-H (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AGGTCTATATAAGCAGAGC
- 3′ sequencing primer TCTACAAATGTGGTATGGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmScarlet-H_peroxisome_C1 was a gift from Dorus Gadella (Addgene plasmid # 85064 ; http://n2t.net/addgene:85064 ; RRID:Addgene_85064) -
For your References section:
mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr. Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. 10.1038/nmeth.4074 PubMed 27869816
