pMTS_mScarlet_N1
(Plasmid #85057)

• Purpose: In vivo visualization of the mitochondria (can be used for colocalization studies)
• Depositing Lab: Dorus Gadella
• Publication: Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.

Backbone

• Vector backbone: pmScarlet_N1
• Backbone manufacturer: Gadella Lab
• Backbone size w/o insert (bp): 4739
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MTS
• Alt name: Mitochondrial Targeting Signal
• Alt name: COX8A
• Species: H. sapiens (human)
• Insert Size (bp): 87
• Mutation: aa1-29
• Entrez Gene: COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
• Promoter: CMV
• Tag / Fusion Protein:
  • mScarlet (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: AGGTCTATATAAGCAGAGC
• 3′ sequencing primer: TCTACAAATGTGGTATGGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid has extra sequence (T7 promoter) between CMV and the insert.