UbcH10 DN
(Plasmid #8505)

• Depositing Lab: Joan Ruderman
• Publication: Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2362-7.

Backbone

• Vector backbone: pT7-7
• Backbone size w/o insert (bp): 2488
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UbcH10
• Alt name: ubiquitin-conjugating enzyme H10
• Alt name: E2-C
• Species: H. sapiens (human)
• Insert Size (bp): 543
• Mutation: C114S, L118S
• Entrez Gene: UBE2C (a.k.a. UBCH10, dJ447F3.2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map. (Ruderman pHSC6.11)