Skip to main content
Addgene

UbcH10
(Plasmid #8504)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8504 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pT7-7
  • Backbone size w/o insert (bp) 2488
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcH10
  • Alt name
    ubiquitin-conjugating enzyme H10
  • Alt name
    E2-C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    543
  • Entrez Gene
    UBE2C (a.k.a. UBCH10, dJ447F3.2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. (Ruderman pHSC2.4)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbcH10 was a gift from Joan Ruderman (Addgene plasmid # 8504 ; http://n2t.net/addgene:8504 ; RRID:Addgene_8504)

  • For your References section:

    Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase. Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV. Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2362-7. 10.1073/pnas.94.6.2362 PubMed 9122200
Related items:
Commonly requested with: