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pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)
(Plasmid #85039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-Ef1a-DIO EYFP (Plasmid #27056)
  • Backbone size w/o insert (bp) 5590
  • Total vector size (bp) 7115
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RFP-P2A-tTA
  • Species
    Entacmaea quadricolor, Picorna virus, E coli, HSV
  • Insert Size (bp)
    1525

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CGAAGTTATGCAGAATGGTAGCTGGATTGTAGCTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova) was a gift from Takuji Iwasato (Addgene plasmid # 85039 ; http://n2t.net/addgene:85039 ; RRID:Addgene_85039)

  • For your References section:

    Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. 10.1038/srep35747 PubMed 27775045
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