CMV-C 197 E7
(Plasmid
#85034)
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PurposeC-Flag/HA tagged HPV 197 E7
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85034 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCMV bam neo
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 6885
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV 197 E7
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SpeciesHPV 197
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Insert Size (bp)285
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MutationCodon optimized for expression
- Promoter CMV
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Tags
/ Fusion Proteins
- Flag (C terminal on backbone)
- HA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer Bglobin-intron-F
- 3′ sequencing primer Bglobin-pA-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOriginal template DNA was synthesized by Genscript as a codon-optimized version of HPV 197 E7.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-C 197 E7 was a gift from Karl Munger (Addgene plasmid # 85034 ; http://n2t.net/addgene:85034 ; RRID:Addgene_85034) -
For your References section:
Proteomic analysis of the gamma human papillomavirus type 197 E6 and E7 associated cellular proteins. Grace M, Munger K. Virology. 2016 Oct 20;500:71-81. doi: 10.1016/j.virol.2016.10.010. 10.1016/j.virol.2016.10.010 PubMed 27771561