CMV-N 197 E6
(Plasmid #85031)

• Purpose: N- Flag/HA tagged HPV 197 E6
• Depositing Lab: Karl Munger
• Publication: Grace et al Virology. 2016 Oct 20;500:71-81. doi: 10.1016/j.virol.2016.10.010.

Backbone

• Vector backbone: CMV bam neo
• Backbone size w/o insert (bp): 6600
• Total vector size (bp): 7044
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HPV 197 E6
• Species: HPV 197
• Insert Size (bp): 444
• Mutation: Codon optimized for expression
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: Bglobin-intron-F
• 3′ sequencing primer: Bglobin-pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original template DNA was synthesized by Genscript as a codon-optimized version of HPV 197 E7.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry