pET-UbcH10-DN
(Plasmid
#8502)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepeT29a
- Backbone size w/o insert (bp) 5242
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUbcH10
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Alt nameubiquitin-conjugating enzyme H10
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SpeciesH. sapiens (human)
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Insert Size (bp)540
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MutationDominant Negative, C114S
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Entrez GeneUBE2C (a.k.a. UBCH10, dJ447F3.2)
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map. For bacterial expression of C-terminal His-tagged UbcH10-wt. (Ruderman pET29-wt)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-UbcH10-DN was a gift from Joan Ruderman (Addgene plasmid # 8502 ; http://n2t.net/addgene:8502 ; RRID:Addgene_8502) -
For your References section:
Cell cycle-regulated proteolysis of mitotic target proteins. Bastians H, Topper LM, Gorbsky GL, Ruderman JV. Mol Biol Cell 1999 Nov;10(11):3927-41. 10.1091/mbc.10.11.3927 PubMed 10564281
Map uploaded by the depositor.
