pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
(Plasmid #85006)

• Purpose: For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.
• Depositing Lab: Takuji Iwasato
• Publication: Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.

Backbone

• Vector backbone: pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)
• Backbone size w/o insert (bp): 8178
• Total vector size (bp): 10264
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-ires-tTA
• Species: Aequorea victoria, Encephalomyocarditis virus, E coli, HSV
• Insert Size (bp): 2086

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EBV_rev_primer

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone plasmid (pK029) is available in Addgene.