pcDNA3-Flag-Hsp40-S171A
(Plasmid
#84999)
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PurposeHsp40/DnaJB1 expression plasmid
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3-Flag
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsp40
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SpeciesH. sapiens (human)
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MutationSerine 171 mutated to Alanine
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Entrez GeneDNAJB1 (a.k.a. HSPF1, Hdj1, Hsp40, RSPH16B, Sis1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The pcDNA3-Flag-Hsp40 wild-type plasmid was obtained from Dr. Muraguchi and is described by Liu QL, Kishi H, Ohtsuka K, Muraguchi A. Blood 2003; 102:1788-1796. PMID:12738667
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Flag-Hsp40-S171A was a gift from Ugo Moens (Addgene plasmid # 84999 ; http://n2t.net/addgene:84999 ; RRID:Addgene_84999)