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pcDNA3-Flag-Hsp40-S149A/S151A
(Plasmid #84998)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84998 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-Flag
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp40
  • Species
    H. sapiens (human)
  • Mutation
    Serine 149 and 151 mutated to Alanine
  • Entrez Gene
    DNAJB1 (a.k.a. HSPF1, Hdj1, Hsp40, RSPH16B, Sis1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pcDNA3-Flag-Hsp40 wild-type plasmid was obtained from Dr. Muraguchi and is described by Liu QL, Kishi H, Ohtsuka K, Muraguchi A. Blood 2003; 102:1788-1796. PMID:12738667

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Flag-Hsp40-S149A/S151A was a gift from Ugo Moens (Addgene plasmid # 84998 ; http://n2t.net/addgene:84998 ; RRID:Addgene_84998)
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