pFLAG-CMV2-Hsp27-S15A/S78A/S82A
(Plasmid
#84997)
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PurposeHsp27 expression plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84997 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFLAG-CMVTM-2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsp27
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SpeciesH. sapiens (human)
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MutationSerine 15 and 78 and 82 mutated to Alanine
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Entrez GeneHSPB1 (a.k.a. CMT2F, HEL-S-102, HMN2B, HMND3, HS.76067, HSP27, HSP28, Hsp25, SRP27)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The pFLAG-CMV2-Hsp27 wild-type plasmid was obtained from Dr. Park and is described by Park KJ, Gaynor RB, Kwak YT. J Biol Chem 2003; 278:35272-35278. PMID:12829720
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG-CMV2-Hsp27-S15A/S78A/S82A was a gift from Ugo Moens (Addgene plasmid # 84997 ; http://n2t.net/addgene:84997 ; RRID:Addgene_84997)
