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Addgene

JBEI-6833
(Plasmid #84983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrc99a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NudB-PMD
  • Species
    S. cerevisiae (budding yeast)
  • Promoter trc promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13_pUC_rev
  • 3′ sequencing primer rrnB-T1-term-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JBEI-6833 was a gift from Taek Soon Lee (Addgene plasmid # 84983 ; http://n2t.net/addgene:84983 ; RRID:Addgene_84983)

  • For your References section:

    Metabolic engineering for the high-yield production of isoprenoid-based C(5) alcohols in E. coli. George KW, Thompson MG, Kang A, Baidoo E, Wang G, Chan LJ, Adams PD, Petzold CJ, Keasling JD, Lee TS. Sci Rep. 2015 Jun 8;5:11128. doi: 10.1038/srep11128. 10.1038/srep11128 PubMed 26052683
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