Skip to main content
Addgene

JBEI-12050
(Plasmid #84982)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBbA5c
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MevTo-T21-MK-aphA
  • Species
    S. cerevisiae (budding yeast)
  • Promoter LacUV5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pBbA5c-5
  • 3′ sequencing primer rrnB-T1-term-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JBEI-12050 was a gift from Taek Soon Lee (Addgene plasmid # 84982 ; http://n2t.net/addgene:84982 ; RRID:Addgene_84982)

  • For your References section:

    Isopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production. Kang A, George KW, Wang G, Baidoo E, Keasling JD, Lee TS. Metab Eng. 2016 Mar;34:25-35. doi: 10.1016/j.ymben.2015.12.002. Epub 2015 Dec 17. 10.1016/j.ymben.2015.12.002 PubMed 26708516
Related items:
Commonly requested with: