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Addgene

JBEI-9314
(Plasmid #84979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrc99a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMD
  • Species
    S. cerevisiae (budding yeast)
  • Promoter trc promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13_pUC_rev
  • 3′ sequencing primer rrnB-T1-term-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JBEI-9314 was a gift from Taek Soon Lee (Addgene plasmid # 84979 ; http://n2t.net/addgene:84979 ; RRID:Addgene_84979)

  • For your References section:

    Isopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production. Kang A, George KW, Wang G, Baidoo E, Keasling JD, Lee TS. Metab Eng. 2016 Mar;34:25-35. doi: 10.1016/j.ymben.2015.12.002. Epub 2015 Dec 17. 10.1016/j.ymben.2015.12.002 PubMed 26708516
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