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Addgene

HOXA9 (murine) FLAG pET
(Plasmid #8494)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8494 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA9
  • Alt name
    FLAG-Hoxa9
  • Alt name
    HOX1G
  • Alt name
    HOX1.7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1048
  • Mutation
    FLAG epitope tag replaces T7 tag in the pET backbone; ribosomal binding site; makes protein by Western
  • GenBank ID
    NM_010456
  • Entrez Gene
    Hoxa9 (a.k.a. D6a9, Hox-1.7)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Debra Wolgemuth

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA9 (murine) FLAG pET was a gift from Corey Largman (Addgene plasmid # 8494 ; http://n2t.net/addgene:8494 ; RRID:Addgene_8494)

  • For your References section:

    AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, Buchberg AM, Largman C. Mol Cell Biol 1997 Nov;17(11):6448-58. PubMed 9343407
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