hGli1 flag3x
(Plasmid #84922)

• Purpose: mammalian expression of Gli1
• Depositing Lab: Martin Fernandez-Zapico
• Publication: Fernandez-Zapico lab plasmids (unpublished)

Backbone

• Vector backbone: C-Terminal p3xFLAG-CMV14
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gli1
• Alt name: GLI family zinc finger 1
• Species: H. sapiens (human)
• Insert Size (bp): 3318
• Entrez Gene: GLI1 (a.k.a. GLI, PAPA8, PPD1)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFlag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry